Can you teach an employee to be autonomous or take initiative?
Autonomy and initiative largely come from a person's internal intention — if someone wants to achieve big things, you can coach them on how; but if they just want to do their job and go home, you can't instill ambition in them, and that's okay too.
How do you build confidence for high-pressure public situations like panels?
Get comfortable admitting what you don't know rather than panicking or bluffing; know your strengths and weaknesses clearly, and respond to unfamiliar topics with confidence by simply saying you don't have expertise there rather than trying to fake knowledge.
How do you decide whether to say yes or no to an opportunity or request?
Use a 'fuck yeah or fuck no' filter — if it's not an enthusiastic yes, treat it as a no, because you only have limited energy/capacity ('fucks to give') and overcommitting to things you feel obligated to do takes away from what you're actually passionate about.
How can you say no to requests (e.g. speaking engagements) without feeling guilty or burning bridges?
Rather than flatly declining, redirect the person to other capable people who can fulfill the need — e.g., providing a list of other women entrepreneurs for speaking requests — so everyone still gets a good outcome.